منابع مشابه
Total internal reflection fluorescence.
Total internal reflection fluorescence (TIRF) is an optical effect particularly well-suited to the study of molecular and cellular phenomena at liquid/solid interfaces. Such interfaces are central to a wide range of biochemical and biophysical processes: binding to and triggering of cells by hormones, neurotransmitters, and antigens; blood coagulation at foreign surfaces; electron transport in ...
متن کاملTotal internal reflection Raman spectroscopy.
Total internal reflection (TIR) Raman spectroscopy is an experimentally straightforward, surface-sensitive technique for obtaining chemically specific spectroscopic information from a region within approximately 100-200 nm of a surface. While TIR Raman spectroscopy has long been overshadowed by surface-enhanced Raman scattering, with modern instrumentation TIR Raman spectra can be acquired from...
متن کاملSingle-reflection attenuated total reflection of organic monolayers on silicon.
Attenuated total re ection (ATR) spectroscopy, a wellknown surface technique, is frequently used for examining very thin lms on substrates. However, when it comes to examining thin lms on silicon substrates, the high refractive index of the substrates seems to preclude ATR analysis. This paper demonstrates that extraordinary sensitivity can be achieved by using ATR to analyze monolayers on...
متن کاملTotal internal reflection fluorescence (TIRF) microscopy.
Total internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical technique that provides for the excitation of fluorophores in an extremely thin axial region ("optical section"). The method is based on the principle that when excitation light is totally internally reflected in a transparent solid (e.g., coverglass) at its interface with liquid, an electromagnetic field, ca...
متن کاملTotal internal reflection: a deeper look.
In the present paper, we have presented a Maxwellian boundary-type solution for total internal reflection with unbounded incident waves at an interface between two nonabsorbing media, in which the instantaneous, time varying, and time averaged radiant fluxes have been determined at all points in the two media. Solutions for the s and p polarizations were found for which the instantaneous tangen...
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ژورنال
عنوان ژورنال: Physical Review (Series I)
سال: 1905
ISSN: 1536-6065
DOI: 10.1103/physrevseriesi.21.346